On October 8, 2013, about 1210 eastern daylight time, a Piper PA46-350P JetProp, N640BD, operated by Municipal Code Corporation, sustained minor damage when its nose landing gear collapsed while landing at the Tallahassee Regional Airport (TLH), Tallahassee, Florida. The airline transport pilot was not injured. Visual meteorological conditions prevailed and an instrument flight rules flight plan had been filed for the flight that departed Sarasota-Bradenton International Airport (SRQ), Sarasota, Florida, destined for Gulfport, Mississippi. The business flight was conducted under the provisions of Title 14 Code of Federal Regulations Part 91.The pilot reported that the takeoff from SRQ was normal; however, after he retracted the landing gear, the red landing gear warning annunciator light remained illuminated. He cycled the landing gear and observed three corresponding green annunciator lights when the gear handle was selected to the extended position, but he continued to observe the red landing gear annunciator warning light after the landing gear was selected to the retracted position. The pilot elected to divert to TLH, which was where the airplane was based. He again received cockpit annunciator light indications that the all three landing gear were down and locked prior to landing; however, during touchdown, the nose of the airplane continued to slowly drop until the propeller contacted the runway. Subsequent inspection of the airplane revealed that the aft nose landing gear actuator bolt was fractured. The bolt was installed through mounts on either side of the actuator fitting and through a spherical bearing in the upper fitting for the actuator. The bolt head with about 1-inch of the shank was not recovered. The remaining portion of the bolt, which included the locknut and a washer was retained and forwarded to the NTSB Materials Laboratory, Washington, DC, for examination. Review of maintenance records revealed that the airplane had been operated for about 2,850 hours since it was modified under a Supplemental Type Certificate (STC) by Rocket Engineering Corporation, Spokane, Washington, on August 13, 2001. The STC called for replacing the original factory installed Lycoming TIO-540 series engine with a Pratt & Whitney Canada PT6A-34 series engine, and included associated airframe alterations. Examination of the retained components by an NTSB metallurgist revealed that the nose landing gear actuator attachment separated due to the fracture of the bolt at the upper attachment fitting from reverse-bending fatigue. The head of the bolt was missing, which precluded verification of the bolt part number. The installed bolt contained a drilled shank, which was inconsistent with the specified part number of a NAS6607-38 bolt; however, the bolt diameter in the grip, hardness, and material composition conformed to the required specification. The fracture and wear patterns on the bolt were consistent with some level of clamping force applied during installation; however, the level of applied torque was unknown and could not be determined. An evaluation of the loading spectrum for the nose landing gear attachment and a conversation with a representative from the STC holder revealed that tug loads could be a significant contribution to the overall load profile. In response to an inquiry regarding the frequency that a tug was used to move the incident airplane, a representative of the owner indicated that the airplane took 180 to 270 trips per year, and a tug was utilized approximately 75 percent of the time. [Additional information can be found in the NTSB Materials Laboratory Factual Report No. 14-017 located in the public docket.] After the airplane was repaired and returned to service, maintenance personnel stated that the bolt required a washer to be installed to occupy a gap between the actuator and the engine mounts. The washer was installed prior to the incident, and when properly torqued, there were no gaps present.